COMPOSITE TRANSDUCERS FOR AMPEROMETRIC BIOSENSORS - THE GLUCOSE SENSOR

A new concept of a composite transducer for amperometric biosensors ba sed on the use of a solid substance with amphiphilic character (called a solid binding matrix, SBM) is presented. The electrochemical proper ties of the transducers prepared with five different SBMs and the char acteristics and performance of SBM-based glucose sensors prepared by t hree different methods are described, Biosensor stability is evaluated and discussed. The biosensor was used for the determination of glucos e in wine, yielding results which were consistent with those obtained with the commercially available Glucose Enzyme Photometric Kit. The av erage accuracy was 6% for the whole range of analyzed concentrations ( 0.2-47 g/L) using the same sample dilution in a buffer.