SIMULTANEOUS SOLID-PHASE EXTRACTION, DERIVATIZATION, AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC QUANTIFICATION OF THROMBOXANE AND PROSTACYCLIN METABOLITES, PROSTAGLANDINS, AND ISOPROSTANES IN URINE
J. Wubert et al., SIMULTANEOUS SOLID-PHASE EXTRACTION, DERIVATIZATION, AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC QUANTIFICATION OF THROMBOXANE AND PROSTACYCLIN METABOLITES, PROSTAGLANDINS, AND ISOPROSTANES IN URINE, Analytical chemistry, 69(11), 1997, pp. 2143-2146
The current analytical methods for the various prostanoids require a s
eparate and extended sample workup, derivatization, and gas chromatogr
aphic/mass spectrometric detection of each compound, Therefore, we dev
eloped and validated a rapid method for the common purification, deriv
atization, and GC/MS determination of 11-dehydrothromboxane B-2, 2,3-d
inor-6-keto-PGF(1a), PGF(2a), PGE(2), PGD(2), and isoprostanes in urin
e. A single reversed-phase solid-phase extraction step and modified re
action conditions yielded excellent sample purification at high recove
ries and efficient derivatization for all compounds in one vial. The m
ethod allows, for the first time, the simultaneous quantification of t
hese index metabolites of systemic thromboxane and prostacyclin synthe
sis, renal prostaglandin formation, and nonenzymatic in vivo lipid per
oxidation in a single GC/MS run with high sensitivity and precision.