SIMULTANEOUS SOLID-PHASE EXTRACTION, DERIVATIZATION, AND GAS-CHROMATOGRAPHIC MASS-SPECTROMETRIC QUANTIFICATION OF THROMBOXANE AND PROSTACYCLIN METABOLITES, PROSTAGLANDINS, AND ISOPROSTANES IN URINE

The current analytical methods for the various prostanoids require a s eparate and extended sample workup, derivatization, and gas chromatogr aphic/mass spectrometric detection of each compound, Therefore, we dev eloped and validated a rapid method for the common purification, deriv atization, and GC/MS determination of 11-dehydrothromboxane B-2, 2,3-d inor-6-keto-PGF(1a), PGF(2a), PGE(2), PGD(2), and isoprostanes in urin e. A single reversed-phase solid-phase extraction step and modified re action conditions yielded excellent sample purification at high recove ries and efficient derivatization for all compounds in one vial. The m ethod allows, for the first time, the simultaneous quantification of t hese index metabolites of systemic thromboxane and prostacyclin synthe sis, renal prostaglandin formation, and nonenzymatic in vivo lipid per oxidation in a single GC/MS run with high sensitivity and precision.