Antifungal and antibacterial activities were detected in the hemolymph and
gut contents of the cattle tick, Boophilus microplus. A peptide with antiba
cterial activity from the tick gut contents was purified to homogeneity by
reversed-phase chromatography. The molecular mass of the purified peptide w
as 3,205.7 Da, measured by matrix-assisted laser desorption/ionization mass
spectrometry. The amino acid sequence was obtained by Edman degradation an
d showed that the peptide was identical to a fragment of the bovine cu-hemo
globin. A synthetic peptide based on the sequence obtained showed character
ization data identical to those of the isolated material, confirming its st
ructure. The synthetic peptide was active in micromolar concentrations agai
nst Gram-positive bacteria and fungi. These data led us to conclude that th
e antibacterial activity detected in tick gut contents is the result of enz
ymatic processing of a host protein, hemoglobin. This activity may be used
by ticks as a defense against microorganisms.