A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry

Authors
Shiraishi, T Fukusaki, E Kajiyama, S Kobayashi, A
Citation
T. Shiraishi et al., A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry, J CHROMAT A, 855(1), 1999, pp. 337-340
Citations number
10
Categorie Soggetti
Chemistry & Analysis","Spectroscopy /Instrumentation/Analytical Sciences
Journal title
JOURNAL OF CHROMATOGRAPHY A → ACNP
Volume
855
Issue
1
Year of publication
1999
Pages
337 - 340
Database
ISI
SICI code
Abstract
A new method using GC-MS was devised for the convenient measurement of form ate dehydrogenase (FDH) activity in crude tissue samples. FDH activity was detected by measuring headspace (CO2)-C-13, which was enzymically converted from [C-13]formic acid. This method proved to be sensitive and simple for the estimation of FDH activity without complicated pretreatment. (C) 1999 P ublished by Elsevier Science B.V. All rights reserved.