P. Capitan et al., Gas chromatographic-mass spectrometric analysis of stable isotopes of cysteine and glutathione in biological samples, J CHROMAT B, 732(1), 1999, pp. 127-135
A gas chromatographic-mass spectrometric (GC-MS) procedure for the determin
ation of stable isotope labelled glutathione has been applied to animal and
human samples. The method, based on preparation of the N,S-ethoxycarbonyl
methyl eater derivative of the intact peptide, is rapid and requires little
or minor tissue treatment. The same method was applied to cysteine. The me
thod was found to be reliable in terms of within-day and between-day precis
ion, accuracy and linearity. The procedure was applied in humans and animal
s to determine in vivo the glutathione fractional synthesis rate using labe
lled cysteine infusion, The glutathione fractional synthesis rate was found
to be 22.5%/day in blood from a healthy volunteer and 337+/-29%/day in rat
liver. (C) 1999 Elsevier Science BN. All rights reserved.