Tc. Dowling et Rf. Frye, Determination of famotidine in human plasma and urine by high-performance liquid chromatography, J CHROMAT B, 732(1), 1999, pp. 239-243
An improved, rapid and specific high-performance liquid chromatographic ass
ay was developed for the determination of famotidine in human plasma and ur
ine. Plasma samples were alkalinized and the analyte and internal standard
(cimetidine) extracted with water-saturated ethyl acetate, The extracts wer
e reconstituted in mobile phase, and injected onto a C-18 reversed-phase co
lumn; UV detection was set at 267 nm. Urine samples were diluted with nine
volumes of a mobile phase-internal standard mixture prior to injection. The
lower limits of quantification in plasma and urine were 75 ng/ml and 1.0 m
u g/ml, respectively; intra- and inter-day coefficients of variation were l
ess than or equal to 10.5%. This method is currently being used to support
renal function studies assessing the use of intravenously administered famo
tidine to characterize cationic tubular secretion in man. (C) 1999 Elsevier
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