Hybrid Capture (R): A sensitive signal amplification-based chemiluminescent test for the detection and quantitation of human viral and bacterial pathogens
Jg. Lazar et al., Hybrid Capture (R): A sensitive signal amplification-based chemiluminescent test for the detection and quantitation of human viral and bacterial pathogens, J CLIN LIG, 22(2), 1999, pp. 139-151
Signal amplification coupled with a dioxetane chemiluminescent substrate pr
ovides a highly sensitive assay system that has been applied to the detecti
on and quantitation of viral and bacterial nucleic acids. Hybrid Capture(R)
is a universal nucleic acid detection technology that utilizes long genomi
c probes and antibodies specific for RNA:DNA hybrids to achieve a high leve
l of signal amplification by virtue of the fact that each copy of target ca
n bind hundreds or thousands of enzyme labels, The enzyme labels produce ch
emiluminescence by the dephosphorylation of dioxetane-based substrates. Hyb
rid Capture signal amplification assays for the detection of human papillom
avirus, cytomegalovirus, Chlamydia trachomatis, Neisseria gonorrhoeae, hepa
titis B virus, and human immunodeficiency virus have been developed and dem
onstrate performance comparable to target amplified methods while providing
improved sample handling and a simpler assay format.