Optimizing culture of Chlamydia pneumoniae by using multiple centrifugations

Authors
Pruckler, JM Masse, M Stevens, VA Gang, L Yang, YH Zell, ER Dowell, SF Fields, BS
Citation
Jm. Pruckler et al., Optimizing culture of Chlamydia pneumoniae by using multiple centrifugations, J CLIN MICR, 37(10), 1999, pp. 3399-3401
Citations number
12
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
JOURNAL OF CLINICAL MICROBIOLOGY
ISSN journal
00951137 → ACNP
Volume
37
Issue
10
Year of publication
1999
Pages
3399 - 3401
Database
ISI
SICI code
0095-1137(199910)37:10<3399:OCOCPB>2.0.ZU;2-7
Abstract
Three methods for the recovery of Chlamydia pneumoniae from spiked nasophar yngeal and blood specimens, including extended culture and additional centr ifugations, were compared. Additional centrifugations and a 7-day culture t ime resulted in a 500- to 5,000-fold increase in the number of detectable i nclusion-forming units.