Artefactual pyruvate and 2-oxobutyrate produced by trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of oxygen

Authors
Mamer, OA Choiniere, L Boismenu, D Lepine, F
Citation
Oa. Mamer et al., Artefactual pyruvate and 2-oxobutyrate produced by trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of oxygen, J INH MET D, 22(7), 1999, pp. 821-826
Citations number
4
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
JOURNAL OF INHERITED METABOLIC DISEASE
ISSN journal
01418955 → ACNP
Volume
22
Issue
7
Year of publication
1999
Pages
821 - 826
Database
ISI
SICI code
0141-8955(199910)22:7<821:APA2PB>2.0.ZU;2-7
Abstract
Trimethylsilylation of methylmalonic and ethylmalonic acids in the presence of headspace atmospheric oxygen is shown to produce the trimethylsilyl der ivatives of pyruvic and 2-oxobutyric acids, along with 2-hydroxy-2-methylma lonic and 2-hydroxy-2-ethylmalonic acids, respectively. This may lead to ov erestimation of these keto acids, if they were not oximated in the original sample, and the mistaken reporting of the 2-hydroxymalonates.