Epstein-Barr virus-encoded RK-BARF0 protein expression

Authors
Kienzle, N Buck, M Greco, S Krauer, K Sculley, TB
Citation
N. Kienzle et al., Epstein-Barr virus-encoded RK-BARF0 protein expression, J VIROLOGY, 73(10), 1999, pp. 8902-8906
Citations number
18
Categorie Soggetti
Microbiology
Journal title
JOURNAL OF VIROLOGY
ISSN journal
0022538X → ACNP
Volume
73
Issue
10
Year of publication
1999
Pages
8902 - 8906
Database
ISI
SICI code
0022-538X(199910)73:10<8902:EVRPE>2.0.ZU;2-9
Abstract
The cellular localization of the Epstein-Barr virus-encoded RK-BARF0 protei n was analyzed by fluorescence microscopy and immunoblotting, The recombina nt RK-BARF0 protein was found to be tightly bound to nuclear structures, wh ereas 16- to 20-kDa RK-BARF0 derivatives, generated by differential splicin g of the RK-BARF0 transcript, were present throughout the cell. Moreover, a previously generated anti-RK-BARF0 rabbit serum was found to cross-react w ith cellular proteins, showing that the previously identified 30- to 35-kDa membrane-associated proteins do not represent RK-BARF0.