A light-inducible Myb-like gene that is specifically expressed in red Perilla frutescens and presumably acts as a determining factor of the anthocyanin forma

The Myb-p1 gene was isolated by screening for differentially expressed Myb- related genes in red (anthocyanin-producing) and green (anthocyanin nonprod ucing) forms of Perilla frutescens. Expression of Myb-p1 is increased 10-fo ld in the red relative to the green form of P. frutescens, and the gene is induced by light. MYB-P1 has only one DNA-binding region, which corresponds to repeat III in the general structure of MYB proteins. In the yeast two-h ybrid system, it was shown that MYB-P1 interacted with MYC-RP, a MYC-relate d transcriptional regulatory protein involved in the control of anthocyanin biosynthesis in P. frutescens. In yeast, MYB-Pl was able to bind to a dihy droflavonol reductase (DFR) gene promoter isolated from red P. frutescens. These data suggest that Myb-p1 may be involved in the regulation of anthocy anin biosynthesis and could therefore be responsible for determining anthoc yanin formation in red P. frutescens.