Use of the Actinobacillus actinomycetemcomitans leukotoxin promoter to drive expression of the green fluorescent protein in an oral pathogen

Authors
Lippmann, JE Froeliger, EH Fives-Taylor, PM
Citation
Je. Lippmann et al., Use of the Actinobacillus actinomycetemcomitans leukotoxin promoter to drive expression of the green fluorescent protein in an oral pathogen, ORAL MICROB, 14(5), 1999, pp. 321-325
Citations number
32
Categorie Soggetti
Microbiology
Journal title
ORAL MICROBIOLOGY AND IMMUNOLOGY
ISSN journal
09020055 → ACNP
Volume
14
Issue
5
Year of publication
1999
Pages
321 - 325
Database
ISI
SICI code
0902-0055(199910)14:5<321:UOTAAL>2.0.ZU;2-O
Abstract
The gene for the green fluorescent protein, gfp, was cloned, under the cont rol of the Actinobacillus actinomycetemcomitans leukotoxin (ltx) promoter, in the A. actinomycetemcomitans shuttle vector, pSU20. A. actinomycetemcomi tans containing the ltx-gfp construct emitted bright green fluorescence in the standard invasion assay using epifluorescence microscopy. These data de monstrate that the green fluorescent protein will be a useful tool for the live analysis of A. actinonmycetemcomitans interactions with host cells, an d that the ltx promoter can be used to drive the expression of non-A, actin omycetemcomitans genes.