M. Saruwatari et al., Low temperature-induced accumulation of eicosapentaenoic acids in Marchantia polymorpha cells, PHYTOCHEM, 52(3), 1999, pp. 367-372
The composition of fatty acids in suspension-cultured cells from the liverw
ort Marchantia polymorpha L. grown at 25 and 15 degrees C was analyzed. The
liverwort cells grown at 25 degrees C contained approximately 18% linoleni
c acid (18:3 omega 3), 11% arachidonic acid (20:4 omega 6) and 3% eicosapen
taenoic acid (20:5 omega 3) as percentages of total fatty acids. When the c
ells were grown at 15 degrees C, the relative amounts of 18:3 and 20:5 show
ed a large increase, while those of other fatty acids were less affected. S
ubcellular fractionation experiments showed that the levels of 20:4 and 20:
5 were increased in the chloroplast fraction but not in the extrachloroplas
t fraction, while the level of 18:3 was increased in both fractions. Lipid
analysis revealed that galactolipids were present in chloroplasts, PE (phos
phatidylethanolamine) in the extrachloroplast fraction and PC (phosphatidyl
choline), PG (phosphatidylgiycerol), PI (phosphatidylinositol) and DAG (dia
cylglycerol) in both compartments. Of lipid species bound to 20:5, only MGD
G (monogalactosyldiacylglycerol) and chloroplastic PC were increased by low
temperature. (C) 1999 Elsevier Science Ltd. All rights reserved.