Microbial transformation of ent-kaur-16-en-19-oic acid was carried out with
R. stolonifer. After seven days of incubation, two metabolites, ent-7 alph
a-hydroxy-kaur-16-en-19-oic acid and ent-12 beta-hydroxy-kaur-9(11),16-dien
-19-oic acid, were isolated as a result of hydroxylation and hpdroxylation/
dehydrogenation, respectively. Incubation for 15 days also afforded ent-16
beta,17-dihydroxy-kauran-19-oic acid. The metabolites were identified by sp
ectroscopic methods. (C) 1999 Elsevier Science Ltd, All rights reserved.