Molecular characterization of alpha,beta-poly(asparthylhydrazide) a new synthetic polymer for biomedical applications

alpha,beta-Poly(asparthylhydrazide) (PAHy) is a new synthetic polymer that exhibits interesting properties and is a candidate for biomedical applicati ons. In this article the characterization of PAHy polymer by multi-angle la ser light scattering (MALS) and single-capillary viscometer (SCV) detectors on-line to a size exclusion chromatography (SEC) system is reported. The S EC-MALS-SCV system furnishes exhaustive and consistent characterization of the PAHy polymer. Further, it is possible to characterize the PAHy polymer through conventional SEC and universal calibration. The universal calibrati on method gives intrinsic viscosity and dispersity very close to those meas ured by the absolute detectors; instead the weight-average molar mass is ap proximately 8% lower. This finding means that the chromatographic separatio n of PAHy polymer is fundamentally based on its hydrodynamic volume. By the SEC-MALS-SCV system the constants of the Mark-Houwink-Sakurada equation we re also estimated. (C) 1999 Elsevier Science Ltd. All rights reserved.