Production of transgenic tropical maize with cryIAb and cryIAc genes via microprojectile bombardment of immature embryos

To enhance the level of resistance to insects in tropical maize germplasm w e have developed techniques to successfully transform elite tropical maize inbred based on the activity of specific cryI proteins against four major m aize pests - corn earworm, fall armyworm, southwestern corn borer and sugar cane borer. Constructs containing cryIAb or cryIAc synthetic genes were use d. To generate transgenic plants we have established methods for biolistic bombardment and the selection and regeneration of immature embryos and call i from the elite tropical lines CML72, CML216, CML323, CML327 and hybrids. Transgenic plants resistant to the herbicide Basta(TM) contained the bands for the cry, bar and gus genes as detected by Southern blot analyses. A sim ple leaf bioassay presented varying levels of resistance to Southwestern co rn borer of transgenic tropical maize carrying the cryIAc gene. Analyses of the progenies confirmed the sexual transmission of the introduced genes an d their stable expression.