M. Uze et al., Single-stranded DNA in the genetic transformation of wheat (Triticum aestivum L.): transformation frequency and integration pattern, THEOR A GEN, 99(3-4), 1999, pp. 487-495
Two non-linked marker genes (gus and bar) were co-introduced by microprojec
tile bombardment into wheat cells. Four different DNA structures were compa
red with respect to ability to integrate into the wheat genome: circular or
linear (1) DNA as a single- or double-stranded plasmid (ss and ds, respect
ively). In eight independent experiments, linearized DNA integrated in the
ds or ss form with a high efficiency of up to 14% for 1-ssDNA. Molecular an
alyses by Southern blotting showed that all DNA forms gave a similar compli
cated integration pattern of the bar gene.