Mc. Voss et al., Species differentiation of slugs (Mollusca, Pulmonata) by using DNA-fingerprinting techniques (SSR-a PCR; RAPD), Z PFLANZENK, 106(4), 1999, pp. 387-391
Citations number
14
Categorie Soggetti
Plant Sciences
Journal title
ZEITSCHRIFT FUR PFLANZENKRANKHEITEN UND PFLANZENSCHUTZ-JOURNAL OF PLANT DISEASES AND PROTECTION
The differentiation of slug species within or between genera using morpholo
gical features can be difficult and sometimes inaccurate. So far the method
s of dissecting specimen and using the reproductive tract as a characterist
ic (KERNEY et al. 1983) as well as protein electrophoresis (BACKELJAU et al
. 1996) have been described as feasible approaches eo determine slug specie
s. In this contribution the utility of DNA-fingerprinting using either simp
le sequence repeat anchored polymerase chain reaction (SSR-a PCR) or random
amplified polymorphic DNA (RAPD) is portrayed. DNA samples for both method
s were isolated from fresh somatic slug tissue and several oligonucleotide
primers have been used to generate DNA fingerprints. PCR products were reso
lved by agarose gel electrophoresis and visualized by ultraviolet transillu
mination. In this paper it is shown that both met:hods are suitable molecul
ar tools to distinguish the slug species Arion distinctus, Arion fasciatus
and Deroceras reticulatum on the species and the genera level.