The structure of the bulbus arteriosus of the Antarctic teleost, Trematomus
bernacchii, has been studied by light, scanning, and transmission electron
microscopy. The wall of the bulbus arteriosus is divided into endocardial,
subendocardial, middle and external layers. The endocardial endothelium co
vers the inner surface of the bulbus wall and invaginates into the subendoc
ardium to form solid epithelial cords that show secretory activity. The sub
endocardial tissue is divided into finger-like ridges. Ridge cells located
under the endocardium appear in niches limited by collagen fibers and thin
cell extensions. Away from the endocardium ridge cells cluster into small g
roups, show some of the characteristics of smooth muscle cells, and appear
enmeshed in a filamentous meshwork that lacks collagen and elastin fibers.
The middle bulbus layer is formed by typical smooth muscle cells that are e
nmeshed in a filamentous meshwork similar to that observed in the ridges. T
he ridges and the middle layer appear to be formed by the same cell type, s
mooth muscle, with a gradient of differentiation from the endocardium towar
d the middle layer. In the absence of elastin fibers the filamentous meshwo
rk should confer elastic properties to the bulbus wall. The stretching of t
he meshwork along the main axis of the middle layer cells, and between diff
erent cellular layers, suggests the existence of tensile stress and, hence,
the involvement of smooth muscle cells in bulbus wall dynamics. The extern
al layer is formed by numerous cellular types embedded in a collagenous mat
rix. Among these cellular types, myofibroblasts, macrophages, granulocytes,
lymphocytes, dendrite-like cells, degenerating cells, and plasma cells can
be recognized. The subepicardial tissue appears to be a specialized site i
nvolved in the production of the humoral immune response and displays many
of the morphological characteristics of a germinal center. The outer limiti
ng layer of the bulbus, the visceral pericardium, is formed by epithelial c
ells that show desmosomes and tight junctions. This suggests a close contro
l of permeability with respect to the pericardial fluid. Anat Rec 256:116-1
26, 1999. (C) 1999 Wiley-Liss, Inc.