The amounts of protein measured by absorbance at 280 nm, succinyl-L-alanyl-
L-alanyl-L-prolyl-L-leucine-p-nilroanilide (Suc-Ala-Ala-Pro-Leu-pNA), d-val
yl-cyclohexyl-alanyl-L-arginine-p-nitroanilide (Val-CHA-Arg-pNA), and gluta
myl-L-phenylalanine-p-nitroanilide (Glu-Phe-pNA) amidolytic activities, and
prostate-specific antigen (PSA) were measured in human seminal plasma (HSP
) samples separated from the semen of 46 cases, including 13 cases of azoos
permia and 33 cases of normozoospermia showing either good or poor quality
of liquefaction. There was a highly significant correlation between the con
centrations of all amidolytic enzyme activities studied and the concentrati
on of PSA in HSP samples (p < .01). The HSP sample volume showed a relative
ly good negative coefficient of correlation to all items measured (p < .01)
with the exception of protein concentration. The Suc-Ala-Ala-Pro-Leu-pNA,
Val-CHA-Arg-pNA, and Glu-Phe-pNA amidolytic activities in azoospermia HSP s
amples were 2.33, 1.68, and 1.43 times higher, respectively, than those of
normozoospermia samples showing good quality liquefaction. After the additi
on of morphologically purified human sperm to HSP sample of azoospermia cas
es, the Suc-Ala-Ala-Pro-Leu-pNA amidolytic activity in the HSP sample of az
oospermia was not decreased for up to 18 h incubation, while the number of
motile human sperm gradually declined, and no motile human sperm were detec
ted after 18 h of incubation. The high Suc-Ala-Ala-Pro-Leu-pNA amidolytic a
ctivity in HSP samples of azoospermia cases did not result From a lack of m
otility.