Effects of chlorpromazine on actin polymerization: Slackening of filament elongation and filament annealing

Citation
A. Milzani et I. Dalledonne, Effects of chlorpromazine on actin polymerization: Slackening of filament elongation and filament annealing, ARCH BIOCH, 369(1), 1999, pp. 59-67
Citations number
37
Categorie Soggetti
Biochemistry & Biophysics
Journal title
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
ISSN journal
00039861 → ACNP
Volume
369
Issue
1
Year of publication
1999
Pages
59 - 67
Database
ISI
SICI code
0003-9861(19990901)369:1<59:EOCOAP>2.0.ZU;2-O
Abstract
We have analyzed the effect of chlorpromazine (CPZ) on pure actin. We have found that CPZ quenches Trp-79 and Trp-86 fluorescence and, in agreement wi th an earlier report on conventional actin, inhibits actin polymerization, lowering the extent of polymerization. Moreover, novel polymerization data are presented indicating that CPZ decreases the maximum polymerization rate in a dose-dependent manner. The assembly inhibition results from the slack ening of oligomer formation during the early stages of polymerisation, of f ilament elongation and of filament annealing, Finally, CPZ strongly inhibit s actin filament network formation. (C) 1999 Academic Press.