A. Milzani et I. Dalledonne, Effects of chlorpromazine on actin polymerization: Slackening of filament elongation and filament annealing, ARCH BIOCH, 369(1), 1999, pp. 59-67
We have analyzed the effect of chlorpromazine (CPZ) on pure actin. We have
found that CPZ quenches Trp-79 and Trp-86 fluorescence and, in agreement wi
th an earlier report on conventional actin, inhibits actin polymerization,
lowering the extent of polymerization. Moreover, novel polymerization data
are presented indicating that CPZ decreases the maximum polymerization rate
in a dose-dependent manner. The assembly inhibition results from the slack
ening of oligomer formation during the early stages of polymerisation, of f
ilament elongation and of filament annealing, Finally, CPZ strongly inhibit
s actin filament network formation. (C) 1999 Academic Press.