Rh. Oliver et al., Induction of apoptosis in luteinized granulosa cells by the MAP kinase kinase (MEK) inhibitor PD98059, BIOC BIOP R, 263(1), 1999, pp. 143-148
Citations number
36
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Our objective is to test the hypothesis that inhibition of mitogen-activate
d protein (MAP) kinase kinase (MEK) with PD98059 in human luteinized granul
osa cells will block epidermal growth (EGF)-stimulated MAP kinase activity
and induce apoptosis, Luteinized granulosa cells from human in vitro fertil
ization aspirates were cultured and treated with the following: (1) vehicle
; (2) PD98059; (3) EGF; (4) PD98059 + EGF. Treatment with PD98059 suppresse
d MAP kinase activity, inhibited MAP kinase phosphorylation by Western blot
analysis, blocked nuclear translocation of phosphorylated MAP kinase by co
nfocal microscopy, and increased percentages of subdiploid apoptotic nuclei
by flow cytometry, Our data are the first evidence that a relationship may
exist between the MAP kinase pathway and control of apoptosis in human lut
einized granulosa cells. These results support the hypothesis that suppress
ion of the MAP kinase pathway may lead to apoptosis in these cells. (C) 199
9 Academic Press.