9-[(2 ' S,3 ' S)-3 '-formyl-2 ',3 '-dihydroxypropyl]adenine: A facile affinity-labeling probe of human S-adenosyl-L-homocysteine hydrolase

Authors
Kitade, Y Nakanishi, M Yatome, C
Citation
Y. Kitade et al., 9-[(2 ' S,3 ' S)-3 '-formyl-2 ',3 '-dihydroxypropyl]adenine: A facile affinity-labeling probe of human S-adenosyl-L-homocysteine hydrolase, BIOORG MED, 9(18), 1999, pp. 2737-2740
Citations number
18
Categorie Soggetti
Chemistry & Analysis
Journal title
BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
ISSN journal
0960894X → ACNP
Volume
9
Issue
18
Year of publication
1999
Pages
2737 - 2740
Database
ISI
SICI code
0960-894X(19990920)9:18<2737:9'S'S'>2.0.ZU;2-5
Abstract
Treatment of human recombinant S-adenosyl-L-homocysteine (SAH) hydrolase wi th 9-[(2'S,3'S)-3'-formyl-2',3'-dihydroxypropyl]adenine (FDHPA) caused irre versible inactivation in a time- and concentration-dependent manner (K-i = 8.8 mu M, k(inact) = 0.09 min(-1)).. FDHPA behaved as a facile affinity-lab eling probe of SAH hydrolase. (C) 1999 Elsevier Science Ltd. Air rights res erved.