Clinical scale isolation of highly purified peripheral CD34(+) progenitorsfor autologous and allogeneic transplantation in children

Citation
P. Lang et al., Clinical scale isolation of highly purified peripheral CD34(+) progenitorsfor autologous and allogeneic transplantation in children, BONE MAR TR, 24(6), 1999, pp. 583-589
Citations number
20
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
Journal title
BONE MARROW TRANSPLANTATION
ISSN journal
02683369 → ACNP
Volume
24
Issue
6
Year of publication
1999
Pages
583 - 589
Database
ISI
SICI code
0268-3369(199909)24:6<583:CSIOHP>2.0.ZU;2-H
Abstract
We present our experience with three clinical scale isolation methods for p ositive selection of CD34(+) progenitors from peripheral blood for autologo us and allogeneic transplantation in children, A combination of the CellPro device and the Magnetic Activated Cell Sorting system (MACS), as well as t wo different combinations of MACS systems were used (VarioMACS-SuperMACS an d SuperMACS-SuperMACS). With the CellPro-MACS combination (16 separations), a median purity of 96.2% and a median recovery of 42% CD34(+) cells could be achieved, whereas the two step MACS systems (55 and 29 separations) show ed a median purity of 97.6% and 98.0% and a median recovery of 96.5% and 97 %, respectively. Depletion of T cells was profound (4-5 log). A total of 34 patients in the autologous and 18 patients in the allogeneic setting have been transplanted with highly enriched CD34(+) cells, obtained by these met hods. Only one patient failed to engraft, all other patients showed a rapid and sustained hematological engraftment with the longest follow-up of 3 ye ars. In summary, especially the two step MACS systems have proven to be app ropriate tools for enrichment of CD34(+) cells, yielding both high purity a nd good recovery, and can thus be used for tumor cell purging in the autolo gous setting and for effective T cell depletion in the allogeneic setting.