P. Lang et al., Clinical scale isolation of highly purified peripheral CD34(+) progenitorsfor autologous and allogeneic transplantation in children, BONE MAR TR, 24(6), 1999, pp. 583-589
Citations number
20
Categorie Soggetti
Hematology,"Medical Research Diagnosis & Treatment
We present our experience with three clinical scale isolation methods for p
ositive selection of CD34(+) progenitors from peripheral blood for autologo
us and allogeneic transplantation in children, A combination of the CellPro
device and the Magnetic Activated Cell Sorting system (MACS), as well as t
wo different combinations of MACS systems were used (VarioMACS-SuperMACS an
d SuperMACS-SuperMACS). With the CellPro-MACS combination (16 separations),
a median purity of 96.2% and a median recovery of 42% CD34(+) cells could
be achieved, whereas the two step MACS systems (55 and 29 separations) show
ed a median purity of 97.6% and 98.0% and a median recovery of 96.5% and 97
%, respectively. Depletion of T cells was profound (4-5 log). A total of 34
patients in the autologous and 18 patients in the allogeneic setting have
been transplanted with highly enriched CD34(+) cells, obtained by these met
hods. Only one patient failed to engraft, all other patients showed a rapid
and sustained hematological engraftment with the longest follow-up of 3 ye
ars. In summary, especially the two step MACS systems have proven to be app
ropriate tools for enrichment of CD34(+) cells, yielding both high purity a
nd good recovery, and can thus be used for tumor cell purging in the autolo
gous setting and for effective T cell depletion in the allogeneic setting.