Transglycosylation of cellobiose by partially purified Trichoderma viride cellulase

A commercial cellulase from Trichoderma viride was fractionated into three fractions, F1, F2, and F3, in order to investigate transglycosylation activ ities. Among these fi actions, F3, which demonstrated highly hydrolytic act ivity toward p-nitrophenyl P-D-glucopyranoside and Avicel, most effectively catalyzed the transglycosylation of cellobiose and converted cellobiose in to beta-Glc-(1 --> 6)-beta-glc-(1 --> 4)-Glc and beta-Glc-(1 --> 6)-beta-Gl c-(1 --> 6)-beta-Glc-(1 --> 4)-Glc. The F3 fraction contained the enzyme to catalyze beta-glucosyl transfer toward only the C-6 position of the sugar acceptor, and thus it is expected to be of use for syntheses of functional oligosaccharides. (C) 1999 Elsevier Science Ltd. All rights reserved.