Chemical structure of a polysaccharide from Campylobacter jejuni 176.83 (serotype O : 41) containing only furanose sugars

A neutral polysaccharide was obtained by hot phenol-water extraction of bio mass from Campylobacter jejuni 176.83 and subsequently separated from acid- liberated core oligosaccharide of lipopolysaccharide by sequential GPC on B io-Gel P6 and TSK-40 columns. All sugar components of the trisaccharide rep eating unit of the polysaccharide were found to be of the furanose ring for m. The major trisaccharide contained B-L-arabinose, 6-deoxy-P-D-altro-hepto se (beta-Ddd-altHep) and 6-deoxy-beta-L-altrose (beta-L-6d-Alt), whereas in the minor trisaccharide the beta-L-6d-Alt is replaced by its C-5 epimer al pha-D-Fuc. On the basis of H-1 and C-13 NMR spectroscopic studies, includin g 2D ROESY, HMQC and HMQC-TOCSY experiments, the following structures of th e repeating units were established: -->2)-beta-L-Araf-(1 --> 2)-beta-D-6d-altHepf-(1 --> 2)-beta-L-6d-Altf-(1 - -> (75%) -->2)-beta-L-Araf-(1 --> 2)-beta-D-6d-altHepf-(1 --> 2)-alpha-D-Fu cf-(1 --> (25%) (C) 1999 Elsevier Science Ltd. All rights reserved.