A collagen network formation effector from leaves of Premna subscandens

As a part of the search for biologically active plant products, M cells, wh ich form a collagen fiber network in vitro after a prolonged culture period , were used. The n-BuOH-soluble fraction of a methanol extract of leaves of Premna subscandens exhibited promotion of collagen network formation by M cells. Extensive isolation work guided by a bioassay afforded a phenylethan oid, acteoside, as an active compound.