CHEMILUMINESCENCE AND ANTIOXIDANT LEVELS DURING PEROXISOME PROLIFERATION BY FENOFIBRATE

Citation
Sl. Arnaiz et al., CHEMILUMINESCENCE AND ANTIOXIDANT LEVELS DURING PEROXISOME PROLIFERATION BY FENOFIBRATE, Biochimica et biophysica acta. Molecular basis of disease, 1360(3), 1997, pp. 222-228
Citations number
40
Categorie Soggetti
Biology,Biophysics
ISSN journal
09254439
Volume
1360
Issue
3
Year of publication
1997
Pages
222 - 228
Database
ISI
SICI code
0925-4439(1997)1360:3<222:CAALDP>2.0.ZU;2-M
Abstract
Fenofibrate, the hypolipidemic drug and peroxisome proliferator, was g iven to mice (0.23% w/w in the diet) during 1-3 weeks and H2O2 and TBA RS steady state concentrations, liver chemiluminescence and antioxidan t levels were measured. Administration of fenofibrate during 2 weeks i nduced an increase of 89% in H2O2 steady state concentration. Spontane ous chemiluminescence was decreased by 57% during fenofibrate treatmen t, while no significant effect was observed on TBARS concentration. Hy droperoxide-initiated chemiluminescence was decreased by 56% after 15 days of fenofibrate treatment, probably due to an increase in endogeno us antioxidant levels. Total and oxidized glutathione increased gradua lly after fenofibrate administration, obtaining maximal increases of 6 7% and 58% respectively, after 22 days of treatment. An increase of 55 % was found in ubiquinol levels in treated mice, as compared with the controls. alpha-tocopherol content was decreased by 51% in the liver o f fenofibrate-treated mice. According to our findings, the high rate o f H2O2 production associated with peroxisome proliferation, would not lead to an increase in lipid peroxidation. This can be explained by th e presence of high levels of ubiquinols, which act as an antioxidant. The increased production of H2O2, would lead to DNA damage directly, a nd not through lipid peroxidation processes.