T. Yasukawa et al., Imaging of photosynthetic and respiratory activities of a single algal protoplast by scanning electrochemical microscopy, CHEM LETT, (9), 1999, pp. 975-976
Scanning electrochemical microscopy (SECM) based on the reduction current f
or oxygen was used for imaging of respiratory and photosynthetic activities
of single, living protoplasts. In the dark, the image of the protoplast ap
peared as a spot with lower oxygen concentration due to consumption of oxyg
en by respiration. Under light irradiation, the protoplast appeared as the
opposite image because it generated oxygen by photosynthesis. SECM images c
learly displayed a decrease in photosynthetic activity of the protoplast in
jected with 3-(3,4-dichlorophenyl)- 1, 1-dimethylurea (DCMU), a photosynthe
tic inhibitor.