Enantioseparations with the macrocyclic antibiotic ristocetin A using a countercurrent process in CE

The chiral selectivity of ristocetin A was examined in a countercurrent pro cess in CE using a coated column to suppress electroosmotic now. Excellent enantioseparations of several nonsteroidal antiinflammatories, dansylamino acids, dinitrophenyl-derivatized amino acids, and other optically active co mpounds were achieved. The chiral selectivity of ristocetin A also was exam ined as a function of antibiotic concentration and pH. Enantioresolution wa s found to significantly improve with a slight increase in migration time u pon increasing chiral selector concentration. Enantioselectivities were fou nd to be greatly influenced by pH of the running buffer. (C) 1999 Wiley-Lis s, Inc.