Identification of differentially expressed genes in Con A-activated Carp (Cyprinus carpio L.) leucocytes

A cDNA library was constructed from the message RNA (mRNA) obtained from Co n A-induced head kidney (HK) leucocytes of carp (Cyprinus carpio L.). Diffe rential screening of the cDNA was carried out by hybridization against the total cDNA probes from normal, Con A-uninduced HK leucocytes or Con A-induc ed HK leucocytes of carp. The differential expression patterns of certain c DNA clones were confirmed by Southern-blot and Northern-blot analysis. Sing le-pass of the sequencing analysis and homology search in Genbank (EMBL) re vealed those differentially expressed cDNA clones encode for cytochrome c o xidase sub-unit II and III (COII and COIII), elongation factor-1 beta (EF-1 beta), bleomycin hydrolase (BH), heat shock cognate protein 70 (HSC70) and 16S ribosomal RNA (16S rRNA). (C) 1999 Elsevier Science Inc. All rights re served.