Production of leukotriene B-4 and prostaglandin E-2 by turbot (Scophthalmus maximus) leukocytes

Production of two eicosanoids derived from lipoxygenase and cyclooxygenase activities: leukotriene B-4 (LTB4) and prostaglandin E-2 (PGE(2)), respecti vely, have been simultaneously determined in turbot (Scophthalmus maximus) blood leucocyte and kidney macrophage supernatants by a reverse phase high performance liquid chromatography (HPLC) system coupled with a Diode-Array detector. Levels of LTB4 after calcium ionophore challenge were 4.08 ng ml( -1) in blood leukocyte supernatants and 0.25 ng ml(-1) in kidney macrophage supernatants. The levels found for PGE(2) were 428.23 and 606.67 ng ml(-1) in blood leukocytes and kidney macrophage supernatants, respectively. When blood leukocytes were treated with the respective inhibitors for the enzym es implicated on the synthesis of both compounds an inhibition of 90.35% wa s observed for PGE(2) and 76.44% for LTB4. The detection limit of the metho d was 0.15 ng ml(-1) for LTB4 and 50 ng ml(-1) for PGE(2). (C) 1999 Elsevie r Science Inc. All rights reserved.