Measuring the complexity of cell cycle arrest and killing of drugs: Kinetics of phase-specific effects induced by Taxol

Background: Paclitaxel (Taxol) is known to act mainly in mitosis, interferi ng with microtubule dynamics, but effects on the other cells cycle phases h ave been reported also. However, a comparative picture of perturbation and killing in the G(1), S and G(2)M phases after drug treatment is lacking. Th e approach developed by our group tackles the problem of the complexity of cell cycle effects with the aid of a computer program simulating cell cycle progression and new quantities measuring cell-cycle arrest and death. Methods: The program generates data that were compared with those given by absolute cell counts and by different flow cytometry techniques, enabling u s to follow the fate of G1 and G2M blocked cells either re-entering the cyc le or dying, distinguishing cytostatic and cytotoxic effects. Apoptosis was analyzed in order to refine the description of cytotoxic effects. Results: We estimated the number of blocked and dead cells after short-term Taxol treatments in a range of concentrations and post-drug incubation tim es. G2M block was immediately active at low concentrations but was reversib le, becoming irreversible only at the highest concentrations. G1 block beca me active later, allowing cell cycle progression of cells initially in G1, but was still active 48 h post-treatment, at intermediate concentrations. S -phase delay was detected after 24 h. The death rate was much higher within G1 than G2M blocked cells. Conclusions: Our analysis unraveled the complexity of cell cycle effects of the drug, and revealed the activity of G1 checkpoint, hidden by a prompter but less cytotoxic G(2)M block. Cytometry 37:113-124, 1999. (C) 1999 Wiley -Liss, Inc.