Oligonucleotide-modified electrodes for fast electron transfer to cytochrome c

Gold electrodes were modified with short ds-oligonucleotides via thiol bind ing to form a thin and stable surface layer. The modification was character ised by impedance measurements and used as a promoter for fast electron tra nsfer to cytochrome c. The protein was investigated both immobilised and in solution showing reversible electrochemical behaviour in each case. The mo dification proved to have a good adsorption capability for the redox protei n which was also found to be reversible. In the immobilised state at the el ectrode cytochrome c reacted with superoxide radicals in solution, exemplif ied by cyclic voltammetric measurements. (C) 1999 Elsevier Science S.A. All rights reserved.