A new self-assembled modified electrode for competitive immunoassay

Alternating films constructed upon successive deposition of redox polymer O s(bpy)(2)ClPyCH(2)NHpoly (allylamine) and antibiotin IgG were developed for amperometric immunosensor design. Cyclic voltammetric measurements were us ed to verify charge transport between redox sites and the redox surface con centration was estimated upon voltammetric peak integration. Biotin-antibio tin complex formation was evaluated using horseradish peroxidase as an enzy me label. Redox mediation between the modified electrode surface and the re dox site in the enzyme was verified after substrate addition. Multilayer mo dified gold electrodes with biologically active antibiotin IgG molecules we re employed for the development of a competitive immunoassay with electroch emical label detection. (C) 1999 Elsevier Science S.A. All rights reserved.