The diet of detritivorous fish was analysed at four distinct habitats in a
tropical river during the wet and dry seasons. Two methodologies were used
to determine the diet of the fish: (1) the direct analysis of the stomach c
ontents; and (2) the use of stable carbon isotopes. Both methods indicated
food partitioning among the fish. The stomach contents showed what the fish
had ingested when captured and the C-13 isotope indicated what it had assi
milated over a long period of time. The C-13 isotope suggested the existenc
e of sectional differences in the feeding of the detritivorous fish along t
he river. Plants employing the C-4 photosynthesis pathways seem to have lit
tle influence on the diet of these fish. This method proved to be more appr
opriate for the detection of the main food sources of the captured fish.