Several culture, cryopreservation, freezing and thawing methods were tested
to develop an efficient technique for storing cervid and ovine leukocytes.
The best results were obtained with McCoy or Vega y Martinet (VYM) solutio
n with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP
) as cryopreservatives. The best protocol for freezing was 4 degrees C for
30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still froz
en material was placed onto cultivation medium for propagation.