A leukocyte cryopreservation technique for cytogenetic studies

Authors
Duarte, JMB Ramalho, MFPD de Lima, VFH Jorge, W
Citation
Jmb. Duarte et al., A leukocyte cryopreservation technique for cytogenetic studies, GENET MOL B, 22(3), 1999, pp. 399-400
Citations number
7
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENETICS AND MOLECULAR BIOLOGY
ISSN journal
14154757 → ACNP
Volume
22
Issue
3
Year of publication
1999
Pages
399 - 400
Database
ISI
SICI code
1415-4757(199909)22:3<399:ALCTFC>2.0.ZU;2-1
Abstract
Several culture, cryopreservation, freezing and thawing methods were tested to develop an efficient technique for storing cervid and ovine leukocytes. The best results were obtained with McCoy or Vega y Martinet (VYM) solutio n with dimethyl sulfoxide (DMSO), horse serum and polyvinylpyrrolidone (PVP ) as cryopreservatives. The best protocol for freezing was 4 degrees C for 30 min followed by 15 min in liquid nitrogen vapor. To thaw, the still froz en material was placed onto cultivation medium for propagation.