M. Gordeziani et al., Energetic significance of plant monooxygenase individual components participating in xenobiotics degradation, INT BIO BIO, 44(1), 1999, pp. 49-54
Data obtained in this work shows that the transformation of plant cytochrom
e P-450 to P-420 under the action of oxygen and oxidative substrates is ass
ociated to substitution of monooxygenase activity by peroxidase. Under thes
e conditions the oxygenase reductase component and the cytochrome bs change
their function to play a role in the respiratory chain. Thus, electrons ar
e transferred from NADPH to the bc(1)-complex of a mitochondrial respirator
y chain via the reductase and the cytochrome bs. In the presence of xenobio
tics the reductase and cytochrome b(5) switch from their energetic to their
detoxification function, supplying cytochrome P-450 with NADPH reducing eq
uivalents. Furthermore, evidence was obtained suggesting that when NADPH is
deficient, electrons are transferred from complex II and III of the mitoch
ondrial respiratory chain to cytochrome P-450. (C) 1999 Published by Elsevi
er Science Ltd. All rights reserved.