THE TROPHOBLAST PRIMARY-CELL CULTURE

An In-Vitro Model For Studying the Physiology and Pathology of the Pla centa:/Purpose of the study presented here was to establish trophoblas t primary cell cultures as an in vitro model. Trophoblasts were isolat ed and immunologically and morphologically controlled. During subseque nt cultivation for several weeks, the secretory activity was measured to obtain information on the vitality of the cell system. Both the mor phological analysis and the comparison of the syncytiotrophoblasts in situ with the isolated trophoblasts, as well as the immunohistochemica l studies and measurement of endocrinal activity, pointed to a high pe rcentage of freshly isolated syncytiotrophoblasts. The model described here is notable for its high proportion of primary isolated syncytiot rophoblasts, whose vitality for several weeks in culture was confirmed by the study.