Quantitation of flavonoid constituents in Citrus fruits

Twenty-four flavonoids have been determined in 66 Citrus species and near-c itrus relatives, grown in the same field and year, by means of reversed pha se high-performance liquid chromatography analysis. Statistical methods hav e been applied to find relations among the species. The F ratios of 21 flav onoids obtained by applying ANOVA analysis are significant, indicating that a classification of the species using these variables is reasonable to pur sue. Principal component analysis revealed that the distributions of Citrus species belonging to different classes were largely in accordance with Tan aka's classification system.