Reversed phase ion-pair liquid chromatographic determination of nicotine in commercial tobacco products. 1. Moist snuff

The official methods for the determination of nicotine in commercial tobacc o products (AOAC, CORESTA) are based on approaches that are not selective f or nicotine (colorimetric measurement, steam distillation, perchloric acid titration), and the availability of published methods based on state-of-the -art chromatographic methods is limited. Reversed phase ion-pair liquid chr omatography has been established as a viable alternative for the analysis o f basic analytes. A reversed phase ion-pair liquid chromatographic method f or the determination of nicotine in commercial tobacco products was develop ed and optimized in separate experiments (Ciolino, L. A.; Turner, J. A.; Mc Cauley, H. A; Smallwood, A. W.; Yi, T. Y. J. Chromatogr. 1999a, 852 (2), 45 1-463). An extensive within-laboratory performance study of the optimized m ethod was subsequently conducted, and results are presented here for the de termination of nicotine in commercial moist snuff. Results for the determin ation of nicotine in commercial cigarettes are presented in a subsequent pa per (Ciolino, L. A.; Fraser, D. B.; Yi, T. Y.; Turner, J. A.; Barnett, D. Y .; McCauley, H. A. J. Agric. Food Chem. 1999b, 47, 3713-3717).