Ma. Bessieres et al., A single-stop purification for glycine betaine determination in plant extracts by isocratic HPLC, J AGR FOOD, 47(9), 1999, pp. 3718-3722
The glycine betaine content of higher plants can be determined by rapid and
simple isocratic highperformance Liquid chromatography on a C-18 column. T
he method described is particularly suitable for a large number of samples.
Starting from a complex dry plant, it consists of a single aqueous extract
ion followed by a purification over a sole cationic ion-exchange resin (AG1
, OH-) before simple HPLC quantification. The single-step purification was
allowed because the AG1, OH- resin removed 80% of the total amino acids con
tained in the extracts. Praline, which interferes with GB HPLC quantificati
on, was completely retained by this column. The optimized method for GB qua
ntification was then validated by comparison with an IK NMR method.