A method for quick measurement of protein binding to unilamellar vesicles

A general method for measuring interaction of Liposome-protein (or potentia lly small molecules) was developed. This method utilizes biotinylated lipos omes to incubate with interactants. Streptavidin-coated paramagnetic resins were then added and the liposomes (along with bound materials) can be quic kly separated under a magnetic field or by low speed centrifugation. Subseq uently, concentration of unbound materials tin the supernatants) can be dir ectly determined. The described method is particularly useful for proteins or compounds that are not very soluble under certain assay conditions. (C) 1999 Elsevier Science BN. All rights reserved.