Construction of recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 for ferulic acid biotransformation to vanillin

Recombinants Pseudomonas putida BO14 and Escherichia coli QEFCA8 capable of ferulic acid biotransformation to vanillin mere constructed using homologo us recombination and a PCR based cloning strategy, respectively. In the liq uid culture of P. putida BO14, 26.81+/-2.30 mu g vanillin ml(-1) of culture filtrate was detected. In the case of recombinant E. coli QEFCA8, 19.37+/- 1.95 mu g vanillin ml(-1) of culture filtrate was detected. Results indicat e that the strains could be useful for the biotechnological production of v anillin, a very important flavoring substance.