Isolation of a mutant of Kluyveromyces sp Y-85 resistant to catabolite repression

The production of inulinase in Kluyveromyces sp. Y-85 is repressed by the c arbon catabolites. To screen for derepression mutants, the strain of Kluyve romyces sp. Y-85 was treated with the mutagenic agent, ethyl methane sulfon ate (EMS). Initially, mutants resistant to catabolite repression by 2-deoxy -D-glucose were isolated. One such mutant, Kluyveromyces sp. Y-85 Kg, was f ound to exhibit stable, 2-fold higher expression of inulinase than the wild -type in glucose-containing medium, indicating that it is a promising strai n for industrial production.