Repeatability and reproducibility of retention data and band profiles on reversed-phase liquid chromatography columns III. Results obtained with Kromasil C-18 columns

The reproducibility of the retention data and the band profiles was investi gated with Kromasil C-18 columns (silica-based monomeric type reversed-phas e packing material). High precision data were obtained and statistically co mpared among five columns from the same batch (column-to-column reproducibi lity) and six columns, one from each of six different batches (batch-to-bat ch reproducibility). These data were acquired under five different sets of chromatographic conditions, for a group of 30 neutral, acidic and basic com pounds selected as probes following an experimental protocol previously des cribed. Data characterizing the retention time, the retention factor, the s eparation factor, the column efficiency and the peak asymmetry for the diff erent probe compounds are reported. Factors describing the silica surface i nteraction with the selected probe compounds, such as the hydrophobic inter action selectivity, the steric selectivity, and the separation factors of b asic compounds at different pH values were also determined. The influence o f the underlying silica on these data and correlations between the chromato graphic and physico-chemical properties of the different batches are discus sed. (C) 1999 Elsevier Science B.V. All rights reserved.