The purpose of the study was to compare the microhardness data of diff
erently eroded enamel surfaces with the wear caused by toothbrushing.
Sixty bovine enamel specimens were polished and prepared for microhard
ness determination. The polished surfaces were covered with tape excep
t for a 1.3 x 10.0 mm window. Fifteen specimens each were stored in an
erosive beverage (Sprite Light(R)) for either 1, 5 or 15 min. Each sp
ecimen was immersed in 10 ml of the beverage. The remaining 15 specime
ns were not eroded. Interferometrical analysis revealed that substance
loss after 15 min of erosion was negligibly low (about 75 nm). In all
specimens Vickers microhardness determinations were conducted on erod
ed (= final hardness) and non-eroded (= initial hardness) enamel surfa
ces. The specimens were subsequently submitted to toothbrushing abrasi
on using a slurry consisting of 1 g non-fluoridated dentifrice in 5 mi
artificial saliva. The total amount of tooth wear due to erosion and
subsequent abrasion was profilometrically evaluated. Statistical analy
sis revealed a significant decrease in hardness and a significant incr
ease in wear with increasing time of storage in the erosive beverage.
Overall regression analysis yielded a statistically significant correl
ation between both initial and final hardness and the amount of tooth
wear. The correlation of abrasion and final hardness could be describe
d by a quadratic mathematical function. It is concluded that the susce
ptibility of eroded enamel to toothbrushing abrasion can be correlated
with Vickers microhardness values, thereby suggesting an overproporti
onate increase of abrasion with decreasing hardness values. (C) 1997 E
lsevier Science Ltd.