Measurement of (1)J(NC ') and (2)J(HNC ') couplings from spin-state-selective two-dimensional correlation spectrum

A method for the measurement of (1)J(NC') and (2)J(HNC') coupling constants from a simplified two-dimensional [N-15, H-1] correlation spectrum is pres ented. The multiplet components of the (1)J(NC') doublet in the indirect di mension and (2)J(HNC') in the direct dimension are separated into two subsp ectra by spin-state-setective filters, Thus each subspectrum contains no mo re peaks than the conventional [N-15, H-1]-HSQC spectrum, Furthermore, the method for the measurement of (1)J(NC') and (2)(HNC') is designed to exploi t destructive relaxation interference (TROSY). The results are verified ;ag ainst the measurements of (1)J(NC') from spin-state-selective [C-13', H-1] correlation spectra recorded with additional sequence described here, (C) 1 999 Academic Press.