Raman spectroscopic study of uridine nucleotides: Structural information and conformational features

Raman spectra of the sodium salts of uridine and deoxyuridine monophosphori c acid, Na-2(3'-UMP). 4H(2)O, Na-2(5-UMP). 7H(2)O and Na-2(5'-dUMP). 5H(2)O , and the cadmium salt, [Cd(5'-UMP)(H2O)(2)]. 2H(2)O, were examined, Severa l spectral differences were observed owing to nucleoside conformational dif ferences in the uridine residue. The spectroscopic characterization of the nucleoside structures was made on the basis of previous x-ray crystal studi es of these compounds. Although the crystal structures of Ca(3'-UMP). 2H(2) O, Sr(3'-UMP). 4H(2)O and Ba(3'-UMP). 4H(2)O also reported here are as yet unknown, their nucleoside structures were inferred from the position of the phosphoester stretching band. These results enabled us to consider some no vel Raman band markers as diagnostic of the C(3')-endo and C(2')-endo uridi ne conformations. Copyright (C) 1999 John Whiley & Sons, Ltd.