As experimentally found by low-magnification holography, both the size of t
he condenser aperture and the excitation of the objective lens strongly mod
ify the object wavefunction of an electron wave. For example, the phase by
electrically charged polystyrene latex spheres attached to a copper grid in
creases with decreasing excitation of the objective lens and/or decreasing
diameter of the condenser aperture. It was also found that a smaller diamet
er of the condenser aperture increases the phase shift by a carbon foil or
magnetic memory cells. In addition, the phase of the image wave is modified
by the lateral position of the aperture, whereas different electron densit
ies of the imaging beam have only minor influence on the detected phase shi
fts. (C) Elsevier Science Inc., 1999. All rights reserved.